Nickel tris (carboxymethyl) ethylene diamine NUPharose Fast Flow

Product Name:	Nickel tris (carboxymethyl) ethylene diamine NUPharose Fast Flow
Alternative Name:	Ni-TED NUPharose FF
Catalog Number:	NRPB58

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Description

Matrix ：Cross-linked agarose, 6%

Ligand ：Triscarboxymethylethylenediamine, precharged with ~60 μmol Ni²⁺/mL

Particle size range： 45~165 μm

Particle size, d50^a ：~90 μm

Dynamic binding capacity, DBC_10% ^b ：≥20 mg His-tag protein/mL

Recommended operating flow velocity^c ：60~300 cm/h

Maximum flow velocity^d：1200 cm/h，0.3 MPa

pH stability ：3~12 (recommended operating, pH), 3~12 (long-term), 2~14 (short-term)

Chemical stability ：

1) Purification buffer: commonly used aqueous buffer, which may contain 10 mM EDTA, 100 mM β-ME or 50 mM DTT.

2) Cleaning in place (CIP) treatment: 1 M NaOH, 1~2 M NaCl, 70% ethanol, 30% isopropanol, 0.1~0.5% non-ionic surfactant.

3) The performance of the resin remains stable after treatment in the following solutions for a specific time: 10 mM NaOH (long-term), 0.1 M hydrochloric acid (24 h), 6 M guanidine hydrochloride (24 h).

Storage & Shipping ：20% ethanol, 2~30 ℃

Shelf life ：5 years

Note: ^aMore than 90% of the microspheres by volume are in this size range; ^bDynamic binding capacity at 10% breakthrough for E. coli expressed recombinant Staphylococcus aureus protein A with a residence time of 6 mins; ^cThe flow rate can meet the use conditions of 2-6 mins residence time for various types of columns; ^d1 cm diameter column and 10 cm bed height using buffers with the same viscosity as water at 20 °C.